Coding

Part:BBa_K4770027:Design

Designed by: Andrea Camí Bonet   Group: iGEM23_Barcelona-UB   (2023-10-12)

NR_Nitrate_Reductase_Level_1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 7394
    Illegal PstI site found at 8912
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3198
    Illegal PstI site found at 7394
    Illegal PstI site found at 8912
    Illegal NotI site found at 8648
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4928
    Illegal BglII site found at 5394
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 7394
    Illegal PstI site found at 8912
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 7394
    Illegal PstI site found at 8912
    Illegal NgoMIV site found at 1265
    Illegal NgoMIV site found at 1447
    Illegal NgoMIV site found at 3673
    Illegal NgoMIV site found at 4480
    Illegal NgoMIV site found at 4513
    Illegal NgoMIV site found at 5210
    Illegal NgoMIV site found at 9389
    Illegal AgeI site found at 4717
  • 1000
    COMPATIBLE WITH RFC[1000]

Source of this part

Position 4: Hygro R cassette BBa_K4770016 Position 5: pCM1 NiR BBa_K4770019 Position 6: mCherry cassette BBa_K4770017 Position 7: mVenus cassette BBa_K4770025

Design considerations

AlgaGenix’s Level Ms are designed to have the same structure and not having to build different pieces with different positions for each transcriptional unit. Each piece has it’s own design considerations that can be looked up on the individual part’s pages.