Coding

Part:BBa_K4770026:Design

Designed by: Andrea Camí Bonet   Group: iGEM23_Barcelona-UB   (2023-10-12)

NR_Nitrate_Reductase_Level_M

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3882
    Illegal PstI site found at 5146
    Illegal PstI site found at 8289
    Illegal PstI site found at 9815
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5726
    Illegal PstI site found at 3882
    Illegal PstI site found at 5146
    Illegal PstI site found at 8289
    Illegal PstI site found at 9815
    Illegal NotI site found at 9551
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 6281
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3882
    Illegal PstI site found at 5146
    Illegal PstI site found at 8289
    Illegal PstI site found at 9815
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3882
    Illegal PstI site found at 5146
    Illegal PstI site found at 8289
    Illegal PstI site found at 9815
    Illegal NgoMIV site found at 1265
    Illegal NgoMIV site found at 1447
    Illegal NgoMIV site found at 3771
    Illegal NgoMIV site found at 4365
    Illegal NgoMIV site found at 4980
    Illegal NgoMIV site found at 6097
    Illegal NgoMIV site found at 10292
    Illegal AgeI site found at 3543
    Illegal AgeI site found at 5856
  • 1000
    COMPATIBLE WITH RFC[1000]

Source of this part

Position 4: Hygro R cassette BBa_K4770016

Position 5: pCM1 NR BBa_K4770018

Position 6: mCherry cassette BBa_K4770017

Position 7: mVenus cassette BBa_K4770025

Design considerations

AlgaGenix’s Level Ms are designed to have the same structure and not having to build different pieces with different positions for each transcriptional unit. Each piece has it’s own design considerations that can be looked up on the individual part’s pages.