Regulatory

Part:BBa_K4770024:Design

Designed by: Andrea Camí Bonet   Group: iGEM23_Barcelona-UB   (2023-10-12)


PNIT_Nitrate_Dependent_Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 197
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design considerations

MoClo kit provides you with sequences with different overhangs so you can choose the one that better fits you design/cloning plan.


Source of this part

Original sequence was obtained from MoClo kit designed by Crozet, et. al (2018)