DNA
Part:BBa_K4769105:Design
Designed by: Kieran Abbott Group: iGEM23_Cambridge (2023-10-10)
gltX promoter 5’ integration sequence for B. subtilis 3610
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
N/A
Source
B. subtilis 3610 genome
Primers for cloning: FW: gcgggagcactttcagc (+ desired overhangs) RV: gttagcctttttgtatcagtactgtc (+ desired overhangs)