DNA

Part:BBa_K4769104:Design

Designed by: Kieran Abbott   Group: iGEM23_Cambridge   (2023-10-10)


gyrA promoter 5’ integration sequence for B. subtilis 3610


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 667
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 667
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 726
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 667
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 667
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 200
    Illegal SapI.rc site found at 625


Design Notes

N/A


Source

B. subtilis 3610 genome

Primers for cloning: FW: gaagcacggacgatcacc (+ desired overhangs) RV: aatatataggtattgcaggcatcgc (+ desired overhangs)


References