Coding
Part:BBa_K4766012:Design
Designed by: Xinyu Wang Group: iGEM23_DUT-China (2023-10-05)
SnoopCatcher-Mi3
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 28
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 28
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 28
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 28
Illegal AgeI site found at 189 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 373
Design Notes
We linked SnoopCatcher to Mi3 so that it can capture proteins connected with SnoopTag.
Source
References
W. Kang, X. Ma, D. Kakarla, H. Zhang, Y. Fang, B. Chen, K. Zhu, D. Zheng, Z. Wu, B. Li, C. Xue, Angew. Chem. Int. Ed. 2022, 61, e202214001; Angew. Chem. 2022, 134, e202214001.
Keeble AH, Yadav VK, Ferla MP, Bauer CC, Chuntharpursat-Bon E, Huang J, Bon RS, Howarth M. DogCatcher allows loop-friendly protein-protein ligation. Cell Chem Biol. 2022 Feb 17;29(2):339-350.e10.
Veggiani G, Nakamura T, Brenner MD, Gayet RV, Yan J, Robinson CV, Howarth M. Programmable polyproteams built using twin peptide superglues. Proc Natl Acad Sci U S A. 2016 Feb 2;113(5):1202-7.