Coding

Part:BBa_K4766012:Design

Designed by: Xinyu Wang   Group: iGEM23_DUT-China   (2023-10-05)


SnoopCatcher-Mi3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 28
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 28
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 28
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 28
    Illegal AgeI site found at 189
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 373


Design Notes

We linked SnoopCatcher to Mi3 so that it can capture proteins connected with SnoopTag.

Source

References

W. Kang, X. Ma, D. Kakarla, H. Zhang, Y. Fang, B. Chen, K. Zhu, D. Zheng, Z. Wu, B. Li, C. Xue, Angew. Chem. Int. Ed. 2022, 61, e202214001; Angew. Chem. 2022, 134, e202214001.

Keeble AH, Yadav VK, Ferla MP, Bauer CC, Chuntharpursat-Bon E, Huang J, Bon RS, Howarth M. DogCatcher allows loop-friendly protein-protein ligation. Cell Chem Biol. 2022 Feb 17;29(2):339-350.e10.

Veggiani G, Nakamura T, Brenner MD, Gayet RV, Yan J, Robinson CV, Howarth M. Programmable polyproteams built using twin peptide superglues. Proc Natl Acad Sci U S A. 2016 Feb 2;113(5):1202-7.