Coding

Part:BBa_K4766011:Design

Designed by: Xinyu Wang   Group: iGEM23_DUT-China   (2023-10-05)


SpyTag-Streptavidin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 124
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Streptavidin connected with SpyTag can be captured by proteins connected with SpyCatcher.

Source

https://www.rcsb.org/structure/7DY0

References

W. Kang, X. Ma, D. Kakarla, H. Zhang, Y. Fang, B. Chen, K. Zhu, D. Zheng, Z. Wu, B. Li, C. Xue, Angew. Chem. Int. Ed. 2022, 61, e202214001; Angew. Chem. 2022, 134, e202214001.