Coding

Part:BBa_K4766004:Design

Designed by: Xinyu Wang   Group: iGEM23_DUT-China   (2023-10-05)


DogCatcher


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 73
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 73
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 73
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 73
    Illegal AgeI site found at 234
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

DogCatcher can be attached to a protein so that it can capture proteins connected with DogTag.

Source

References

Keeble AH, Yadav VK, Ferla MP, Bauer CC, Chuntharpursat-Bon E, Huang J, Bon RS, Howarth M. DogCatcher allows loop-friendly protein-protein ligation. Cell Chem Biol. 2022 Feb 17;29(2):339-350.e10.