DNA

Part:BBa_K4765135:Design

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-10-10)


ribozyme connected: Dsup + H. ex mtSSB + SAHS 33020 + XRCC1 + FEN1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3307
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 5485
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1516
    Illegal NgoMIV site found at 6063
    Illegal AgeI site found at 230
    Illegal AgeI site found at 301
    Illegal AgeI site found at 5169
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2149
    Illegal BsaI.rc site found at 227
    Illegal BsaI.rc site found at 1784
    Illegal BsaI.rc site found at 1865
    Illegal BsaI.rc site found at 4357
    Illegal SapI site found at 1678
    Illegal SapI.rc site found at 956


Design Notes

none


Source

synthetic

References