DNA

Part:BBa_K4765115

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-10-01)


Twister P1 + T7_RBS + FEN1 + stem-loop

contributed by Fudan iGEM 2023

Introduction

This composite part is composed of FEN1 and ribozyme-assisted polycistronic co-expression system：pRAP. FEN1 plays a pivotal role in DNA maintenance, addressing 5' overhanging flaps formed when DNA strands can't bind correctly.

Usage and Biology

We constructed FEN1 into pRAP to endow E. coli with anti-UV capability.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 617
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1195
    Illegal AgeI site found at 301
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//collections
Parameters
protein