DNA

Part:BBa_K4765114

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-10-01)


Twister P1 + T7_RBS + Rv Dsup + stem-loop

contributed by Fudan iGEM 2023

Introduction

This composite part is composed of Rv Dsup and ribozyme-assisted polycistronic co-expression system：pRAP. Dsup is a nucleosome-binding protein that helps protect DNA from radiation damage. It was discovered in the Tardigrade Species Ramazzottius Varieornatus .

Usage and Biology

We constructed Rv Dsup into pRAP to endow E. coli with anti-UV capability.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 230
    Illegal AgeI site found at 301
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 227
    Illegal SapI.rc site found at 956


[edit]
Categories
//collections
Parameters
protein