Coding

Part:BBa_K4765015:Design

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-07-13)


AnAFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 451


Design Notes

We obtained the gene sequence of AnAFP from GenBank and performed codon optimization specifically for Escherichia coli K12.

Source

synthesized

References