Composite

Part:BBa_K4762022:Design

Designed by: Xuan Hang   Group: iGEM23_NWU-CHINA-A   (2023-10-11)


T5_Promoter-RBS-CAT-RBS-SOD-RBS-Elafin-T7_Terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1286
    Illegal XbaI site found at 2337
    Illegal PstI site found at 1119
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1286
    Illegal NheI site found at 1101
    Illegal NheI site found at 2634
    Illegal PstI site found at 1119
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1286
    Illegal BamHI site found at 83
    Illegal BamHI site found at 1536
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1286
    Illegal XbaI site found at 2337
    Illegal PstI site found at 1119
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1286
    Illegal XbaI site found at 2337
    Illegal PstI site found at 1119
    Illegal AgeI site found at 153
    Illegal AgeI site found at 1943
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon-optimized to work in E. coli


Source

The sequence is synthesized by the company

References