Part:BBa_K4762001

CAT（Catalase）

It catalyzes hydrogen peroxide (H2O2) to produce water and oxygen from peroxisome oxidase, thereby protecting cells from the toxic effects of hydrogen peroxide. Promote the growth of T cells, B cells, bone marrow leukemia cells, melanoma cells, mast cell tumor cells and normal and transformed fibroblasts.

Protein Source

In the 19th century, Thénard discovered H2O2 and suspected that its tissue degradation in organisms was the result of a “special” material activity. Schönbein showed that a “ferment” could detoxify H2O2. Later, Loew named the enzyme that could convert H2O2 into water and oxygen as “catalase”.

Protein Activity/Function

Catalytic activity : 2 H2O2 = 2 H2O + O2

Figure 1. The catalytic activity of CAT is derived from https://www.uniprot.org/uniprotkb/P04040/entry # function.

Reaction Mechanism

Catalase is first oxidized to a high-valent iron intermediate, called compound I ( Cpd I ), and then reduced to a resting state by a second H2O2 molecule

Figure 2. The reaction mechanism of CAT, picture from Reference[1],

References

Glorieux C, Calderon PB. Catalase, a remarkable enzyme: targeting the oldest antioxidant enzyme to find a new cancer treatment approach. Biol Chem. 2017 Sep 26;398(10):1095-1108.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1218
  Illegal PstI site found at 1051
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1218
  Illegal NheI site found at 1033
  Illegal PstI site found at 1051
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1218
  Illegal BamHI site found at 15
  Illegal BamHI site found at 1468
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1218
  Illegal PstI site found at 1051
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1218
  Illegal PstI site found at 1051
  Illegal AgeI site found at 85
  
• 1000
  COMPATIBLE WITH RFC[1000]