Plasmid

Part:BBa_K4761424:Design

Designed by: Pinhan Pan   Group: iGEM23_HiZJU-China   (2023-09-30)


pTarget-pykA3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2064
    Illegal XbaI site found at 2070
    Illegal SpeI site found at 1955
    Illegal PstI site found at 2082
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2064
    Illegal NheI site found at 1932
    Illegal SpeI site found at 1955
    Illegal PstI site found at 2082
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2064
    Illegal BglII site found at 2094
    Illegal BamHI site found at 1920
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2064
    Illegal XbaI site found at 2070
    Illegal SpeI site found at 1955
    Illegal PstI site found at 2082
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2064
    Illegal XbaI site found at 2070
    Illegal SpeI site found at 1955
    Illegal PstI site found at 2082
    Illegal NgoMIV site found at 771
    Illegal AgeI site found at 1977
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

References