Plasmid

Part:BBa_K4761103:Design

Designed by: Pinhan Pan   Group: iGEM23_HiZJU-China   (2023-09-27)


pACYCDuet dual expression vector


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 112
    Illegal XbaI site found at 2749
    Illegal PstI site found at 131
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 112
    Illegal NheI site found at 1908
    Illegal PstI site found at 131
    Illegal NotI site found at 149
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 112
    Illegal BglII site found at 305
    Illegal BamHI site found at 106
    Illegal XhoI site found at 354
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 112
    Illegal XbaI site found at 2749
    Illegal PstI site found at 131
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 112
    Illegal XbaI site found at 2749
    Illegal PstI site found at 131
    Illegal NgoMIV site found at 324
    Illegal AgeI site found at 566
    Illegal AgeI site found at 1994
    Illegal AgeI site found at 2317
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

References