Plasmid

Part:BBa_K4761100:Design

Designed by: Pinhan Pan   Group: iGEM23_HiZJU-China   (2023-09-27)


pSA expression vector


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2920
    Illegal EcoRI site found at 3420
    Illegal SpeI site found at 1669
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2920
    Illegal EcoRI site found at 3420
    Illegal SpeI site found at 1669
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2920
    Illegal EcoRI site found at 3420
    Illegal BglII site found at 1687
    Illegal BamHI site found at 2
    Illegal XhoI site found at 3340
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2920
    Illegal EcoRI site found at 3420
    Illegal SpeI site found at 1669
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2920
    Illegal EcoRI site found at 3420
    Illegal SpeI site found at 1669
    Illegal NgoMIV site found at 217
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

One of original plasmids obtained. It was based on the first constructed plasmid in history pSC101 and has very low copy number of approximately one per cell. So copy number optimization is necessary for our research.

Source

References