Coding

Part:BBa_K4760006:Design

Designed by: Xianghai Jiang   Group: iGEM23_SJTU-BioX-Shanghai   (2023-10-10)


SpyCatcher003


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Start codon not included here; a HA-tag is added on the C terminus.


Source

This is an improved version by Keeble et a'. of the original SpyCatcher. We synthesized it according to the most preferred codon usage of E.coli. BL21 strain.

References