Composite
Part:BBa_K4759270:Design
Designed by: Jianghua Chen Group: iGEM23_Jiangnan-China (2023-10-08)
T7-RBS4-hemB-RBS2-hemD-RBS2-hemC
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1068
Illegal EcoRI site found at 1845
Illegal EcoRI site found at 2136
Illegal PstI site found at 553 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1068
Illegal EcoRI site found at 1845
Illegal EcoRI site found at 2136
Illegal PstI site found at 553 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1068
Illegal EcoRI site found at 1845
Illegal EcoRI site found at 2136
Illegal BamHI site found at 1062
Illegal BamHI site found at 1839 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1068
Illegal EcoRI site found at 1845
Illegal EcoRI site found at 2136
Illegal PstI site found at 553 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1068
Illegal EcoRI site found at 1845
Illegal EcoRI site found at 2136
Illegal PstI site found at 553 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 2748
Design Notes
Cloning the genes from E. coli genome and use RBS2 to link them to constructed the plasmid to express hemB 、hemD 、hemC under the same promoter.
Source
E. coli genome