Composite

Part:BBa_K4759269:Design

Designed by: Jianghua Chen   Group: iGEM23_Jiangnan-China   (2023-10-08)


T7-RBS6-pntAB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 714
    Illegal EcoRI site found at 1353
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 714
    Illegal EcoRI site found at 1353
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 714
    Illegal EcoRI site found at 1353
    Illegal XhoI site found at 720
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 714
    Illegal EcoRI site found at 1353
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 714
    Illegal EcoRI site found at 1353
    Illegal NgoMIV site found at 1190
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We cloned pntAB gene to construct the plasmid pACYCDuet-pntAB

Source

References