Composite

Part:BBa_K4759246:Design

Designed by: Jianghua Chen   Group: iGEM23_Jiangnan-China   (2023-10-08)


T7-RBS1-petH-RBS2-petF(D21Y)-linker-GFP1-10


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1336
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1336
    Illegal NotI site found at 1109
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1336
    Illegal BglII site found at 1647
    Illegal BglII site found at 2316
    Illegal BamHI site found at 1330
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1336
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1336
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Site-directed mutagenesis of the petF protein


Source

petH and petF are come from Synechocystis

References