Composite
Part:BBa_K4759211:Design
Designed by: Jianghua Chen Group: iGEM23_Jiangnan-China (2023-10-07)
T7-RBS3-hemH
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 47
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 47
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 47
Illegal NgoMIV site found at 958
Illegal AgeI site found at 103 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We cloned gene hemH to construct the plasmid to express it.
Source
E. coli genome