Composite
Part:BBa_K4759210:Design
Designed by: jianghua Chen Group: iGEM23_Jiangnan-China (2023-10-07)
T7-RBS5-hemA-RBS2-hemL
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1349
Illegal EcoRI site found at 2406 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1349
Illegal EcoRI site found at 2406
Illegal NheI site found at 2116 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1349
Illegal EcoRI site found at 2406
Illegal BglII site found at 331
Illegal BglII site found at 609
Illegal BamHI site found at 1343 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1349
Illegal EcoRI site found at 2406 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1349
Illegal EcoRI site found at 2406
Illegal AgeI site found at 1766
Illegal AgeI site found at 2099 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 263
Design Notes
We constructed the recombinant plasmid pCDFDuet-hemA-hemL to express hemA and hemL in engineered bacteria, hemA and hemL are use RBS2 to link to under the promoter to express.
Source
E.coli genome
References
[1]Sultana, N. Microbial biotransformation of bioactive and clinically useful steroids and some salient features of steroids and biotransformation. Steroids 2018, 136. DOI: 10.1016/j.steroids.2018.01.007.