Composite

Part:BBa_K4759208:Design

Designed by: Jianghua Chen   Group: iGEM23_Jiangnan-China   (2023-10-07)


T7-RBS4-Nus-linker-Olep


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1463
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1623
    Illegal AgeI site found at 1721
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NusA protein (N-utilization substance A) was fused at the N-terminal of the Olep.

Source

References