Part:BBa_K4759203:Experience
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K4759203
Selection of suitable plasmid
First, we selected high-copy plasmids pRSFDuet, medium-high copy plasmids pETDuet and pET28a, and low-copy plasmid pACYCDuet as carriers to express Olep in E. coli, and the results showed that the recombinant strains constructed with high-copy plasmid pRSFDuet as the carrier had the highest expression of p450 enzyme, cell growth rate, and substrate conversion rate.
Fig2-1: Selection of suitable plasmid. WT represents E. coli O1 strain. The blue-filled triangle represents the biomass (OD600). The red hollow triangle represents the conversion rate (%). Values and triangles represent the means and standard deviations of biological triplicates.
User Reviews
UNIQb166879f50d93266-partinfo-00000000-QINU UNIQb166879f50d93266-partinfo-00000001-QINU