Coding

Part:BBa_K4759099:Design

Designed by: Junyuan Shi   Group: iGEM23_Jiangnan-China   (2023-10-02)


pntAB-RBS2-nadK


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 628
    Illegal EcoRI site found at 1267
    Illegal EcoRI site found at 1540
    Illegal PstI site found at 2147
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 628
    Illegal EcoRI site found at 1267
    Illegal EcoRI site found at 1540
    Illegal PstI site found at 2147
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 628
    Illegal EcoRI site found at 1267
    Illegal EcoRI site found at 1540
    Illegal BglII site found at 2072
    Illegal BamHI site found at 1534
    Illegal XhoI site found at 634
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 628
    Illegal EcoRI site found at 1267
    Illegal EcoRI site found at 1540
    Illegal PstI site found at 2147
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 628
    Illegal EcoRI site found at 1267
    Illegal EcoRI site found at 1540
    Illegal PstI site found at 2147
    Illegal NgoMIV site found at 1104
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Connecting the pntAB sequence and the nadK sequence by the RBS 2 sequence, and is transcribed under the regulation of the same promoter


Source

PntAB and nadK are from the E. coli genome

References