Coding
Part:BBa_K4759042:Design
Designed by: Shiyue Zhao Group: iGEM23_Jiangnan-China (2023-10-05)
hemB-RBS2-hemD-RBS2-hemC
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 982
Illegal EcoRI site found at 1759
Illegal EcoRI site found at 2050
Illegal PstI site found at 467 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 982
Illegal EcoRI site found at 1759
Illegal EcoRI site found at 2050
Illegal PstI site found at 467 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 982
Illegal EcoRI site found at 1759
Illegal EcoRI site found at 2050
Illegal BamHI site found at 976
Illegal BamHI site found at 1753 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 982
Illegal EcoRI site found at 1759
Illegal EcoRI site found at 2050
Illegal PstI site found at 467 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 982
Illegal EcoRI site found at 1759
Illegal EcoRI site found at 2050
Illegal PstI site found at 467 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 2662
Design Notes
Connect the hemB, hemD, and hemC via RBS2 for transcription under the regulation of the same promoter
Source
E. coli genome