Coding
Part:BBa_K4759031:Design
Designed by: Mengrui Tao Group: iGEM23_Jiangnan-China (2023-10-02)
T7-RBS4
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We used pETDuet / pRSFDuet as a plasmid vector and expressed OleP / GFP11-linker-OleP and a highly soluble tag under the control of T7-RBS 4.
Source
From RSFDuet plasmids