Coding

Part:BBa_K4759026

Designed by: Mengrui Tao   Group: iGEM23_Jiangnan-China   (2023-10-02)


nadK

A gene coding for NAD kinase, NAD kinase appears to be an allosteric enzyme, with activity tightly coupled to the NADPH/NADP+ and NADH/NAD+ ratios present in the cell. ATP + NAD(+) <=> ADP + H(+) + NADP(+)

Design Notes

Recombinant plasmid recombinant plasmid pACYCDuet-nadK Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 578
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 578
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 503
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 578
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 578
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None