Coding

Part:BBa_K4759018

Designed by: Mengrui Tao   Group: iGEM23_Jiangnan-China   (2023-10-02)


GST

Recombinant protein expression plays an important role in promoting the development of modern biological technology. By using the host to achieve exogenous protein expression, it provides a powerful tool for studying the structure and function of some important proteins, especially the human source proteins. In order to enable subsequent structure-function studies, the obtained recombinant proteins must be soluble, stable, properly folded as well as biologically active. However, in practical studies, these often become obstacles to obtaining an ideal recombinant protein. The solubility of the recombinant protein expression became the primary addressed problem.The most prominent advantage of GST is its ability to generate affinity with fixed glutathione, elute under non-denaturing conditions of 10 mmol/L reduced glutathione, finally achieving the purpose of affinity purification. GST has another beneficial effect on recombinant proteins, which is to reduce degradation from inside the host and increase protein stability. However, some reports show that the soluble effect of GST is not very obvious.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


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Categories
Parameters
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