Composite

Part:BBa_K4757059:Design

Designed by: Marik Müller   Group: iGEM23_Heidelberg   (2023-10-08)


Ps1/Ps2_XylS-K38R-L224Q/Pm


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 212
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 934
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Ps1/Ps2 shows low expression strength with mutated XylS transcription factor, and may need to be replaced with a constitutively active promoter.


Source

Native in Pseudomonas putida KT 2440

References