Coding

Part:BBa_K4757013:Design

Designed by: Marik Müller   Group: iGEM23_Heidelberg   (2023-10-03)


AlkS-epS4-156


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1705
    Illegal EcoRI site found at 1801
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1705
    Illegal EcoRI site found at 1801
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1705
    Illegal EcoRI site found at 1801
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1705
    Illegal EcoRI site found at 1801
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1705
    Illegal EcoRI site found at 1801
    Illegal NgoMIV site found at 219
    Illegal NgoMIV site found at 690
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

AlkS-epS4-156 was not able to be synthesized as one single fragment, instead synthesis had to be subdivided into 3 fragments and then cloned together via Gibson-assembly. The fragments were designed with NEBase changer with a length of ~1000 bp


Source

WT-AlkS is natively found in Pseudomonas Putida (and other Pseudomona species). The part was received by synthesize

References