Coding

Part:BBa_K4757011

Designed by: Marik Müller   Group: iGEM23_Heidelberg   (2023-10-03)


XylS-K38R-L224Q

Based on the paper from Li, Jiawei et al. two point mutations (K38R, L224Q) were introduced in the XylS transcription factor to change the recognition from m-toluic acid to terepthalic acid (TPA), a degradation product of poly ethylene terepthalate (PET).

    1. data###

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 32
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 754
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None