Composite

Part:BBa_K4757007:Design

Designed by: Marik Müller   Group: iGEM23_Heidelberg   (2023-11-16)


pEM7 AlkS-V760E/pAlkB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1753
    Illegal EcoRI site found at 1849
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1753
    Illegal EcoRI site found at 1849
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1753
    Illegal EcoRI site found at 1849
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1753
    Illegal EcoRI site found at 1849
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1753
    Illegal EcoRI site found at 1849
    Illegal NgoMIV site found at 267
    Illegal NgoMIV site found at 738
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The pAlkB promoter does not have to be near the AlkS gene. For our testing it was ~ 1 kb upstream


Source

Obtained by synthesis

References