Part:BBa_K4752007
NCED3-RUBY reporter
Discription
The NCED3 promoter is a specialized regulatory region in the plant genome that becomes active under drought conditions. When a plant experiences drought stress, the NCED3 promoter becomes activated, initiating a cascade of molecular events. This activation, in turn, triggers the expression of the RUBY reporter system.
RUBY serves as a reporter system, generating a visible and distinctive red coloration in the plant's leaves when activated by the NCED3 promoter . The change in leaf coloration is a clear visual indicator, making it easy to identify plants undergoing drought stress. This feature is valuable for monitoring and studying the plant's response to drought conditions, aiding researchers and farmers in assessing and managing plant health.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1378
Illegal XbaI site found at 25
Illegal PstI site found at 1982 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1378
Illegal PstI site found at 1982 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1378
Illegal BglII site found at 509
Illegal BglII site found at 2014
Illegal BglII site found at 5737
Illegal BamHI site found at 1794
Illegal XhoI site found at 178 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1378
Illegal XbaI site found at 25
Illegal PstI site found at 1982 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1378
Illegal XbaI site found at 25
Illegal PstI site found at 1982
Illegal NgoMIV site found at 2340
Illegal NgoMIV site found at 2919
Illegal NgoMIV site found at 4614 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1641
Assembly
The existing pDR5-RUBY plasmid was subjected to enzymatic digestion (enzymes used: SmaI , PstI ), and the digested plasmid was ligated to the corresponding NCED3 promoter using homologous recombination enzymes (ClonExpress II One Step Cloning Kit, Vazyme Biotech). Subsequently, the ligated plasmid was transformed into Escherichia coli for replication and amplification, followed by successful assembly validation through sequencing. The assembled construct was preserved for future use.
Reference
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