Coding

Part:BBa_K4750001:Design

Designed by: Johannes Radde   Group: iGEM23_TU-Dresden   (2023-10-10)


SP+T8 (tpSil3)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 143
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Between the signal peptide and the T8 sequence two amino acids (G and T) were added as a linker. No stop codon is included as it as designed to be used as an N-terminal fusion protein.


Source

Thalassiosira pseudonana

References