Coding

Part:BBa_K4743024:Design

Designed by: Jen Hsien, Liu   Group: iGEM23_PTSH-Taiwan   (2023-10-08)


hph


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 238
    Illegal PstI site found at 328
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 238
    Illegal PstI site found at 328
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 238
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 238
    Illegal PstI site found at 328
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 238
    Illegal PstI site found at 328
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NO


Source

NO

References

1. Gritz, L., & Davies, J. (1983). Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae. Gene, 25(2-3), 179–188. https://doi.org/10.1016/0378-1119(83)90223-8