Part:BBa_K4743022

bkt

Bkt is a gene that is essential for producing astaxanthin. It catalyzes beta-Carotene to Echinenone

Overall metabolic pathway of Astaxanthin

Figure 1. The Matabolic pathway of Astaxanthin production.

References

1. Jin, J., Wang, Y., Yao, M., Gu, X., Li, B., Liu, H., Ding, M., Xiao, W., & Yuan, Y. (2018). Astaxanthin overproduction in yeast by strain engineering and new gene target uncovering. Biotechnology for biofuels, 11, 230. https://doi.org/10.1186/s13068-018-1227-4

2.Zheng, K., Wang, C., Xiao, M., Chen, J., Li, J., & Hu, Z. (2014). Expression of bkt and bch genes from Haematococcus pluvialis in transgenic Chlamydomonas. Science China. Life sciences, 57(10), 1028–1033. https://doi.org/10.1007/s11427-014-4729-8

3.Huang, J., Zhong, Y., Sandmann, G., Liu, J., & Chen, F. (2012). Cloning and selection of carotenoid ketolase genes for the engineering of high-yield astaxanthin in plants. Planta, 236(2), 691–699. https://doi.org/10.1007/s00425-012-1654-6

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1308
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 349