Coding

Part:BBa_K4743018:Design

Designed by: Jen Hsien, Liu   Group: iGEM23_PTSH-Taiwan   (2023-09-16)


DRE (Bacillus Subtilis Optimized)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence is optimized for Bacillus Subtilis

Source

Escherichia phage D6

References

1. Anastassiadis, K., Fu, J., Patsch, C., Hu, S., Weidlich, S., Duerschke, K., Buchholz, F., Edenhofer, F., & Stewart, A. F. (2009). Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice. Disease models & mechanisms, 2(9-10), 508–515. https://doi.org/10.1242/dmm.003087

2. Han, X., Zhang, Z., He, L., Zhu, H., Li, Y., Pu, W., Han, M., Zhao, H., Liu, K., Li, Y., Huang, X., Zhang, M., Jin, H., Lv, Z., Tang, J., Wang, J., Sun, R., Fei, J., Tian, X., Duan, S., … Zhou, B. (2021). A suite of new Dre recombinase drivers markedly expands the ability to perform intersectional genetic targeting. Cell stem cell, 28(6), 1160–1176.e7. https://doi.org/10.1016/j.stem.2021.01.007