Part:BBa_K4743018

DRE (Bacillus Subtilis Optimized)

DRE recombinase targets rox sequences and catalyzes recombination. It is found in E. coli and mammalian cells, and it is a site-specific recombinase(SSR). Research by Konstantinos Anastassiadis et al. has shown that the DRE/rox system does not exhibit crossover recombination with the Cre/lox system. This means that the two systems can be used together to create more complex designs.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

1. Anastassiadis, K., Fu, J., Patsch, C., Hu, S., Weidlich, S., Duerschke, K., Buchholz, F., Edenhofer, F., & Stewart, A. F. (2009). Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice. Disease models & mechanisms, 2(9-10), 508–515. https://doi.org/10.1242/dmm.003087

2. Han, X., Zhang, Z., He, L., Zhu, H., Li, Y., Pu, W., Han, M., Zhao, H., Liu, K., Li, Y., Huang, X., Zhang, M., Jin, H., Lv, Z., Tang, J., Wang, J., Sun, R., Fei, J., Tian, X., Duan, S., … Zhou, B. (2021). A suite of new Dre recombinase drivers markedly expands the ability to perform intersectional genetic targeting. Cell stem cell, 28(6), 1160–1176.e7. https://doi.org/10.1016/j.stem.2021.01.007