Composite

Part:BBa_K4743007:Design

Designed by: Jen Hsien, Liu   Group: iGEM23_PTSH-Taiwan   (2023-09-16)


prsA+ Adh1 terminator with adapter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1167
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1167
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1167
    Illegal BamHI site found at 16
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1167
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1167
    Illegal AgeI site found at 1180
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

AAGGTACCGCAGGTGGGATCC is the forward primer of Adapter 1

TCACCGGTGCAGGTGGAATTC is the reverse primer of Adapter 2

Also, BamHI is included in adapter 1 and EcoRI is included in Adpater 2. These two site can be used for cloning

Source

B. amyloliquefaciens

References

1.Liu, Y., Yasawong, M., & Yu, B. (2021). Metabolic engineering of Escherichia coli for biosynthesis of β-nicotinamide mononucleotide from nicotinamide. Microbial biotechnology, 14(6), 2581–2591. https://doi.org/10.1111/1751-7915.13901

2.Gazzaniga, F., Stebbins, R., Chang, S. Z., McPeek, M. A., & Brenner, C. (2009). Microbial NAD metabolism: lessons from comparative genomics. Microbiology and molecular biology reviews : MMBR, 73(3), 529–541. https://doi.org/10.1128/MMBR.00042-08