Composite

Part:BBa_K4743005

Designed by: Jen Hsien, Liu   Group: iGEM23_PTSH-Taiwan   (2023-09-16)


NadV+ADH1 terminator with adapter

NadV is a nicotinamide phosphoribosyltransferase that make Nicotinamide+ Prpp into NMN.

Overall metabolic pathway of NMN

NadV is involved in the pathway of Astaxanthin production in PTSH-Taiwan's project.

Figure 1. The Matabolic pathway of NMN production.

Length checking of NadV sequence

Figure 2. The legnth of NadV sequence .

References

1. Martin, P. R., Shea, R. J., & Mulks, M. H. (2001). Identification of a plasmid-encoded gene from Haemophilus ducreyi which confers NAD independence. Journal of bacteriology, 183(4), 1168–1174. https://doi.org/10.1128/JB.183.4.1168-1174.2001

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1689
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1689
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1689
    Illegal BglII site found at 515
    Illegal BamHI site found at 16
    Illegal BamHI site found at 367
    Illegal BamHI site found at 975
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1689
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1689
    Illegal AgeI site found at 1702
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None