Composite

Part:BBa_K4743004:Design

Designed by: Jen Hsien, Liu   Group: iGEM23_PTSH-Taiwan   (2023-09-16)


NadE+ADH1 terminator with adaptor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1052
    Illegal SpeI site found at 1045
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1052
    Illegal NheI site found at 747
    Illegal NheI site found at 823
    Illegal SpeI site found at 1045
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1052
    Illegal BamHI site found at 16
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1052
    Illegal SpeI site found at 1045
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1052
    Illegal SpeI site found at 1045
    Illegal AgeI site found at 175
    Illegal AgeI site found at 1065
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

AAGGTACCGCAGGTGGGATCC is the forward primer of Adapter 1

TCACCGGTGCAGGTGGAATTC is the reverse primer of Adapter 2

Also, BamHI is included in adapter 1 and EcoRI is included in Adpater 2. These two site can be used for cloning

Source

Francisella tularensis

References

1. Hughes, K. T., Olivera, B. M., & Roth, J. R. (1988). Structural gene for NAD synthetase in Salmonella typhimurium. Journal of bacteriology, 170(5), 2113–2120. https://doi.org/10.1128/jb.170.5.2113-2120.1988