Part:BBa_K4743004

NadE+ADH1 terminator with adaptor

NadE is an NAD synthase. According to William B. Black et al. , this synthase is also used in turning NaMN into NMN.

Length checking of nadE

We use electrophoresis to check the length of the fragment

Figure 1. The length of NadE.

Overall metabolic pathway of NMN

NadE is involved in the pathway of Astaxanthin production in PTSH-Taiwan's project.

Figure 2. The Matabolic pathway of NMN production.

References

1. Hughes, K. T., Olivera, B. M., & Roth, J. R. (1988). Structural gene for NAD synthetase in Salmonella typhimurium. Journal of bacteriology, 170(5), 2113–2120. https://doi.org/10.1128/jb.170.5.2113-2120.1988

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1052
  Illegal SpeI site found at 1045
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1052
  Illegal NheI site found at 747
  Illegal NheI site found at 823
  Illegal SpeI site found at 1045
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1052
  Illegal BamHI site found at 16
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1052
  Illegal SpeI site found at 1045
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1052
  Illegal SpeI site found at 1045
  Illegal AgeI site found at 175
  Illegal AgeI site found at 1065
  
• 1000
  COMPATIBLE WITH RFC[1000]