Part:BBa_K4743002:Design

NadA+ADH1 terminator with adapter

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1284
  Illegal SpeI site found at 1089
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1284
  Illegal SpeI site found at 1089
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1284
  Illegal BamHI site found at 16
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1284
  Illegal SpeI site found at 1089
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1284
  Illegal SpeI site found at 1089
  Illegal AgeI site found at 1297
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

AAGGTACCGCAGGTGGGATCC is the forward primer of Adapter 1

TCACCGGTGCAGGTGGAATTC is the reverse primer of Adapter 2

Also, BamHI is included in adapter 1 and EcoRI is included in Adpater 2. These two site can be used for cloning

Source

Listeria innocua (serotype 6a)

References

1.Fagnocchi, L., Biolchi, A., Ferlicca, F., Boccadifuoco, G., Brunelli, B., Brier, S., Norais, N., Chiarot, E., Bensi, G., Kroll, J. S., Pizza, M., Donnelly, J., Giuliani, M. M., & Delany, I. (2013). Transcriptional regulation of the nadA gene in Neisseria meningitidis impacts the prediction of coverage of a multicomponent meningococcal serogroup B vaccine. Infection and immunity, 81(2), 560–569. https://doi.org/10.1128/IAI.01085-12

2.Michalowski, C. B., Flachmann, R., Löffelhardt, W., & Bohnert, H. J. (1991). Gene nadA, Encoding Quinolinate Synthetase, Is Located on the Cyanelle DNA from Cyanophora paradoxa. Plant physiology, 95(1), 329–330. https://doi.org/10.1104/pp.95.1.329

3.Christine B. Michalowski, Ralf Flachmann, Wolfgang Löffelhardt, Hans J. Bohnert, Gene nadA, Encoding Quinolinate Synthetase, Is Located on the Cyanelle DNA from Cyanophora paradoxa , Plant Physiology, Volume 95, Issue 1, January 1991, Pages 329–330, https://doi.org/10.1104/pp.95.1.329