Part:BBa_K4743000

HPD(Optimized for B. Subtilis)

The HPD gene encodes for 4-hydroxyphenylpyruvate dioxygenase (HPPD), which is an enzyme involved in the catabolism of the amino acid tyrosine. This enzyme plays a crucial role in the breakdown of tyrosine to produce fumarate and homogentisate in the tyrosine degradation pathway. However, the gene HPD plays another crucial role in team iGEM23_PTSH-Taiwan's research: it catalyzes the conversion of α-ketoisocaproate to β-hydroxy β-methylbutyrate.

Mutations in the HPD gene can lead to a rare inherited disorder known as tyrosinemia type III, which is characterized by the accumulation of 4-hydroxyphenylpyruvate and other metabolites in the body.

Overall metabolic pathway of HMB

We use the gene HPD to let B.Subtilis to produce HMB and KIC

Figure 1. The Matabolic pathway of HMB and KIC production.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 397
  Illegal XhoI site found at 79
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

1.Yang, X., Chen, S. L., Lin, C. S., Liu, L. L., Wang, C. H., & Yun, J. P. (2020). Tyrosine metabolic enzyme HPD is decreased and predicts unfavorable outcomes in hepatocellular carcinoma. Pathology, research and practice, 216(11), 153153. https://doi.org/10.1016/j.prp.2020.153153

2.Zhao, D., Tian, Y., Li, X., Ni, M., Zhu, X., & Jia, L. (2020). Variant analysis of HPD genes from two families showing elevated tyrosine upon newborn screening by tandem mass spectrometry (MS/MS). Journal of pediatric endocrinology & metabolism : JPEM, 33(4), 563–567. https://doi.org/10.1515/jpem-2019-0498

3.https://www.ncbi.nlm.nih.gov/gene/3242