Composite

Part:BBa_K4740003:Design

Designed by: Andreea Panti   Group: iGEM23_CNAS-Romania   (2023-10-06)


promoter J23119+alkS+promoter PalkB+alkB1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2035
    Illegal BglII site found at 3874
    Illegal BamHI site found at 2978
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 788
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 950
    Illegal BsaI.rc site found at 421
    Illegal BsaI.rc site found at 723
    Illegal SapI.rc site found at 3541


Design Notes

This RBS was chosen as it has a high protein production rate.


Source

All subparts are found in the registry.

References