Coding

Part:BBa_K4740000:Design

Designed by: Andreea Panti   Group: iGEM23_CNAS-Romania   (2023-10-06)


alkB1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 976
    Illegal BamHI site found at 80
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 643


Design Notes

The sequence had 2 EcoRI sites, which were substituted so that open reading frame is not altered. Moreover, 30 repeats were found, which were substituted in one of the positions(substitution was done for the second or third nucleotide of the codon).


Source

The part comes from the organism Alcanivorax borkumensis SK2.

References