Coding

Part:BBa_K4739012:Design

Designed by: Huaiyuan Ma   Group: iGEM23_SUSTech-Shenzhen   (2023-10-11)


Tenecteplase, a myocardial peptide drug


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 615
    Illegal EcoRI site found at 1087
    Illegal PstI site found at 1204
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 615
    Illegal EcoRI site found at 1087
    Illegal PstI site found at 1204
    Illegal NotI site found at 886
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 615
    Illegal EcoRI site found at 1087
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 615
    Illegal EcoRI site found at 1087
    Illegal PstI site found at 1204
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 615
    Illegal EcoRI site found at 1087
    Illegal PstI site found at 1204
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. Choose codons that are frequently used in the host organism to ensure efficient translation. 2. Optimize for codons corresponding to abundant tRNAs in the host to enhance translation speed. 3. Limit repetitive DNA sequences that may lead to recombination events and genomic instability.


Source

The sequence is created based on the published amino acid sequence of tenecteplase.

References

1. Davydov, L. Tenecteplase: A Review. Clinical Therapeutics 2001, 23, 982–997, doi:https://doi.org/10.1016/s0149-2918(01)80086-2.