Coding
Part:BBa_K4739012:Design
Designed by: Huaiyuan Ma Group: iGEM23_SUSTech-Shenzhen (2023-10-11)
Tenecteplase, a myocardial peptide drug
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 615
Illegal EcoRI site found at 1087
Illegal PstI site found at 1204 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 615
Illegal EcoRI site found at 1087
Illegal PstI site found at 1204
Illegal NotI site found at 886 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 615
Illegal EcoRI site found at 1087 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 615
Illegal EcoRI site found at 1087
Illegal PstI site found at 1204 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 615
Illegal EcoRI site found at 1087
Illegal PstI site found at 1204 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
1. Choose codons that are frequently used in the host organism to ensure efficient translation. 2. Optimize for codons corresponding to abundant tRNAs in the host to enhance translation speed. 3. Limit repetitive DNA sequences that may lead to recombination events and genomic instability.
Source
The sequence is created based on the published amino acid sequence of tenecteplase.
References
1. Davydov, L. Tenecteplase: A Review. Clinical Therapeutics 2001, 23, 982–997, doi:https://doi.org/10.1016/s0149-2918(01)80086-2.