Coding

Part:BBa_K4739010:Design

Designed by: Huaiyuan Ma   Group: iGEM23_SUSTech-Shenzhen   (2023-10-11)


Parkin, an E3 ubiquitin ligase regulating mitochondrial biology


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 614
    Illegal PstI site found at 973
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 614
    Illegal PstI site found at 973
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 614
    Illegal PstI site found at 973
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 614
    Illegal PstI site found at 973
    Illegal NgoMIV site found at 396
    Illegal AgeI site found at 247
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. Choose codons that are frequently used in the host organism to ensure efficient translation. 2. Optimize for codons corresponding to abundant tRNAs in the host to enhance translation speed. 3. Limit repetitive DNA sequences that may lead to recombination events and genomic instability.


Source

We designed the sequence of this part based on the amino acid sequence of the Parkin protein.

References

1. Mizuno, Y.; Hattori, N.; Mori, H.; Suzuki, T.; Tanaka, K. Parkin and Parkinsonʼs Disease. Current Opinion in Neurology 2001, 14, 477–482, doi:https://doi.org/10.1097/00019052-200108000-00008.